Recombinant human Muscle Phosphofructokinase/PFKM protein

PFKM is regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1, 6-bisphosphate (through PFK-1), fructose 2, 6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Recombinant human PFKM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

6-Phosphofructokinase muscle type, GSD7, PFK-1, PFK1, PFKA, PFKX, 6-Phosphofructokinase, muscle type 6 Phosphofructokinase Muscle Type, EC 2.7.1.11, MGC8699, PFKL, PFKM, PFKP, Phosphofructo 1 Kinase Isozyme A, Phosphofructokinase 1, Phosphofructokinase M, Phosphofructokinase, muscle, Phosphofructokinase, muscle type, Phosphofructokinase, polypeptide X, Phosphohexokinase.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

87.3 kDa (800aa) confirmed by MALDI-TOF

Additionnal Information

Muscle Phosphofructokinase, PFKM, 6-Phosphofructokinase muscle type, GSD7, PFK-1, PFK1, PFKA, PFKX, 6-Phosphofructokinase, muscle type 6 Phosphofructokinase Muscle Type, EC 2.7.1.11, MGC8699, PFKL, PFKM, PFKP, Phosphofructo 1 Kinase Isozyme A, Phosphofructokinase 1, Phosphofructokinase M, Phosphofructokinase, muscle, Phosphofructokinase, muscle type, Phosphofructokinase, polypeptide X, Phosphohexokinase., ATGP0536-10 µg, ATGP0536-20 µg, ATGP0536-50 µg, ATGP0536-100 µg, ATGP0536-250 µg, ATGP0536-500 µg, ATGP0536-1 mg, ATGP0536-10, ATGP0536-20, ATGP0536-50, ATGP0536-100, ATGP0536-250, ATGP0536-500, ATGP0536-1

References & Citations

Martinez-Costa OH., et al. (2007) FEBS Lett. 581 (16) :3033-8. ; ; Kim JW., et al. (2004) Mol Cell Biol. 24 (13) :5923-36.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 0.2 M NaCl, and 20% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_000280

Uniprot Accession Number

P08237

Species

Human

AA Sequence

MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITDEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSV GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF TIRDLQANVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV