Recombinant human RRAS protein

RRAS, also known as ras-related protein R-Ras, belongs to the Ras family which functions in signal transduction pathways. This protein has been shown to interact with RASSF5, NCK1, Bcl-2, ARAF and RALGDS. RRAS can bind GTP and GDP, and it has intrinsic GTPase activity. It also regulates the organization of the actin cytoskeleton. Recombinant RRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein R-Ras, p23, Related RAS viral (r-ras) oncogene homolog, R-Ras, Oncogene RRAS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.3 kDa (235aa) confirmed by MALDI-TOF

Additionnal Information

RRAS, ATGP0534-10 µg, ATGP0534-20 µg, ATGP0534-50 µg, ATGP0534-100 µg, ATGP0534-250 µg, ATGP0534-500 µg, ATGP0534-1 mg, ATGP0534-10, ATGP0534-20, ATGP0534-50, ATGP0534-100, ATGP0534-250, ATGP0534-500, ATGP0534-1

References & Citations

Lowe DG. (1987) Cell. 48 (1) :137-46.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 30% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006261

Uniprot Accession Number

P10301

Species

Human

AA Sequence

MSSGAASGTG RGRPRGGGPG PGDPPPSETH KLVVVGGGGV GKSALTIQFI QSYFVSDYDP TIEDSYTKIC SVDGIPARLD ILDTAGQEEF GAMREQYMRA GHGFLLVFAI NDRQSFNEVG KLFTQILRVK DRDDFPVVLV GNKADLESQR QVPRSEASAF GASHHVAYFE ASAKLRLNVD EAFEQLVRAV RKYQEQELPP SPPSAPRKKG GGCPC