Recombinant human RRAS protein
RRAS, also known as ras-related protein R-Ras, belongs to the Ras family which functions in signal transduction pathways. This protein has been shown to interact with RASSF5, NCK1, Bcl-2, ARAF and RALGDS. RRAS can bind GTP and GDP, and it has intrinsic GTPase activity. It also regulates the organization of the actin cytoskeleton. Recombinant RRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ras-related protein R-Ras, p23, Related RAS viral (r-ras) oncogene homolog, R-Ras, Oncogene RRAS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.3 kDa (235aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lowe DG. (1987) Cell. 48 (1) :137-46.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 30% glycerol, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_006261
Uniprot Accession Number
P10301
Species
Human
AA Sequence
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