Recombinant human SGTA protein

SGTA, also known as SGT or hSGT, is a ubiquitously expressed protein that contains three TPR protein-protein interaction repeats. This protien is believed to function as a component of the androgen receptor (AR) -chaperone-cochaperone complex, acting as a cochaperone involved in androgen signaling. In addition, SGTA functions as a cochaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity. On the basis of its role in apoptosis and androgen signaling, SGTA is a potential candidate for PCOS (polycystic ovary syndrome), a disorder characterized by androgen excess, obesity and menstrual disturbances. Recombinant SGTA protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Small glutamine-rich tetratricopeptide repeat-containing protein alpha, alphaSGT, hSGT, SGT, Small glutamine-rich tetratricopeptide repeat-containing protein alpha Small glutamine rich tetratricopeptide repeat TPR containing alpha, uBP, Vpu binding protein.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

35.1 kDa (321aa) confirmed by MALDI-TOF

Additionnal Information

SGTA, Small glutamine-rich tetratricopeptide repeat-containing protein alpha, alphaSGT, hSGT, SGT, Small glutamine-rich tetratricopeptide repeat-containing protein alpha Small glutamine rich tetratricopeptide repeat TPR containing alpha, uBP, Vpu binding protein., ATGP0533-10 µg, ATGP0533-20 µg, ATGP0533-50 µg, ATGP0533-100 µg, ATGP0533-250 µg, ATGP0533-500 µg, ATGP0533-1 mg, ATGP0533-10, ATGP0533-20, ATGP0533-50, ATGP0533-100, ATGP0533-250, ATGP0533-500, ATGP0533-1

References & Citations

Cziepluch C., et al. (1998) J Virol. 72 (5) :4149-56. ; ; Callahan MA., et al. (1998) J Virol. 72 (6) :5189-97. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_003012

Uniprot Accession Number

O43765

Species

Human

AA Sequence

MDNKKRLAYA IIQFLHDQLR HGGLSSDAQE SLEVAIQCLE TAFGVTVEDS DLALPQTLPE IFEAAATGKE MPQDLRSPAR TPPSEEDSAE AERLKTEGNE QMKVENFEAA VHFYGKAIEL NPANAVYFCN RAAAYSKLGN YAGAVQDCER AICIDPAYSK AYGRMGLALS SLNKHVEAVA YYKKALELDP DNETYKSNLK IAELKLREAP SPTGGVGSFD IAGLLNNPGF MSMASNLMNN PQIQQLMSGM ISGGNNPLGT PGTSPSQNDL ASLIQAGQQF AQQMQQQNPE LIEQLRSQIR SRTPSASNDD QQELEHHHHH H