Recombinant human TSG101 protein

TSG101 (Tumor susceptibility gene 101) protein belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. This protein contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. Mutations and alternative splicing in this protein occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. Recombinant human TSG101 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tumor susceptibility gene 101, ESCRT-I complex subunit TSG101, Tumor susceptibility gene 10, TSG10, VPS23

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.7 kDa (181aa) confirmed by MALDI-TOF

Additionnal Information

TSG101, Tumor susceptibility gene 101, TSG10, VPS23, Tumor susceptibility gene 101 ESCRT I complex subunit TSG101, TSG 10, TSG 101, Tumor susceptibility gene 10, Tumor susceptibility gene 101, Tumor susceptibility protein, Tumor susceptibility protein isoform 3, VPS 23, VPS23., ATGP0531-10 µg, ATGP0531-20 µg, ATGP0531-50 µg, ATGP0531-100 µg, ATGP0531-250 µg, ATGP0531-500 µg, ATGP0531-1 mg, ATGP0531-10, ATGP0531-20, ATGP0531-50, ATGP0531-100, ATGP0531-250, ATGP0531-500, ATGP0531-1

References & Citations

Vardhana S., et al. (2010) Immunity. 32 (4) :531-40. ; ; Popov S., et al. (2009) J Virol. 83 (14) :7185-93.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_006283

Uniprot Accession Number

Q99816

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P