Recombinant human Thioredoxin-1 protein

Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human TXN protein, fused to His-tag at N-terminus, was overexpressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >100 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Product Name Alternative

Thioredoxin, TRX, TRX1, Thioredoxin JW0871, ECK0879, Thioredoxin reductase, FAD/NAD (P) -binding, TRXR.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >100 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Molecular Weight

13.9 kDa (125aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Thioredoxin1, Thioredoxin, TRX, TRX1, Thioredoxin JW0871, ECK0879, Thioredoxin reductase, FAD/NAD (P) -binding, TRXR., ATGP0530-10 µg, ATGP0530-20 µg, ATGP0530-50 µg, ATGP0530-100 µg, ATGP0530-250 µg, ATGP0530-500 µg, ATGP0530-1 mg, ATGP0530-10, ATGP0530-20, ATGP0530-50, ATGP0530-100, ATGP0530-250, ATGP0530-500, ATGP0530-1