Recombinant human Thioredoxin-1 protein
Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human TXN protein, fused to His-tag at N-terminus, was overexpressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Thioredoxin, TRX, TRX1, Thioredoxin JW0871, ECK0879, Thioredoxin reductase, FAD/NAD (P) -binding, TRXR.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
13.9 kDa (125aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Pigiet VP., et al. (1986) Proc Natl Acad Sci uSA. 83 (20) :7643-7. ; ; Lundstrom J., et al. (1990) J Biol Chem. 265 (16) :9114-20.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Redox Proteins
NCBI Accession Number
NP_003320
Uniprot Accession Number
P10599
Species
Human
AA Sequence
MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
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