Recombinant human Hemoglobin zeta/HBZ protein
Hemoglobin subunit zeta (HBZ) is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The HBZ gene includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. Recombinant human HBZ fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Zeta globin, Hemoglobin zeta chain, Hemoglobin zeta, Hemoglobin subunit zeta, HBZ-T1, HBZ2, HBZ1, HBZ 2, HBAZ
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
16.7 kDa (150aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lau ET., et al. (2001) Prenat. Diagn. 21 (7) : 529-39.; ; Kidd RD., et al. (2001) Biochemistry. 40 (51) :15669-75.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 100 mM NaCl, 20% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_005323
Uniprot Accession Number
P02008
Species
Human
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