Recombinant human BPGM protein

Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2, 3-BPG concentration. It is responsible for the catalytic synthesis of 2, 3-Bisphosphoglycerate (2, 3-BPG) from 1, 3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active. Recombinant human BPGM, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

31 kDa (267aa) confirmed by MALDI-TOF

Additionnal Information

BPGM, Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192., ATGP0527-10 µg, ATGP0527-20 µg, ATGP0527-50 µg, ATGP0527-100 µg, ATGP0527-250 µg, ATGP0527-500 µg, ATGP0527-1 mg, ATGP0527-10, ATGP0527-20, ATGP0527-50, ATGP0527-100, ATGP0527-250, ATGP0527-500, ATGP0527-1

References & Citations

Pritlove DC., et al. (2006) Placenta. 27 (8) :924-7; ; Wang Y., et al. (2006) J. Biol. Chem. 281 (51) :39642-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001715

Uniprot Accession Number

P07738

Species

Human

AA Sequence

MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNER HYGALIGLNR EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED QGKVKQAKKL EHHHHHH