Recombinant human Cyclin H protein

Cyclin H, also known as CDK7, is a member of the cyclin-dependent protein kinase family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin H forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK) . Cyclin H is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. Recombinant human Cyclin H, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

39.8 kDa (343aa) confirmed by MALDI-TOF

Additionnal Information

Cyclin H, CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H, ATGP0525-10 µg, ATGP0525-20 µg, ATGP0525-50 µg, ATGP0525-100 µg, ATGP0525-250 µg, ATGP0525-500 µg, ATGP0525-1 mg, ATGP0525-10, ATGP0525-20, ATGP0525-50, ATGP0525-100, ATGP0525-250, ATGP0525-500, ATGP0525-1

References & Citations

Schneider, E., et al. (1998) Oncogene. 17 (21) :2733-41.; ; Yee A., et al. (1995) Canser Res. 55 (24) :6058-62.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 2mM EDTA, 0.1M NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_001230

Uniprot Accession Number

P51946

Species

Human

AA Sequence

MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE NRTCLSQLLD IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD DYVSKKSKHE EEEWTDDDLV ESL