Recombinant human NQO2 protein

NQO2 is a member of the NAD (P) H dehydrogenase (quinone) . The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones. This detoxification process protects cells against quinone-induced oxidative stress, cytotoxicity and mutagenicity. Recombinant human NQO2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

N-ribosyldihydronicotinamide:quinone reductase 2, NMOR2, NAD (P) H menadione oxidoreductase 2, Dioxin-inducible, NAD (P) H quinone dehydrogenase 2, Quinone reductase 2, QR2, DHQV, DIA6, NRH:quinone oxidoreductase 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.1 kDa (251aa) confirmed by MALDI-TOF

Additionnal Information

NQO2, Ribosyldihydronicotinamide dehydrogenase, DHQV, DIA6, QR2, Ribosyldihydronicotinamide dehydrogenase Ox2, EC 1.10.99.2, MGC94180, NAD (P) H dehydrogenase quinone 2, NAD (P) H menadione oxidoreductase 1 dioxin inducible 2, NAD (P) H menadione oxidoreductase 2 dioxin inducible, NMOR2, NQO 2, NRH dehydrogenase, NRH:quinone oxidoreductase 2, OTTHuMP00000015948, OTTHuMP00000015949, OTTHuMP00000015953, Ox 2, QR2, Quinone, Quinone reductase 2, Ribosyldihydronicotinamide dehydrogenase., ATGP0522-10 µg, ATGP0522-20 µg, ATGP0522-50 µg, ATGP0522-100 µg, ATGP0522-250 µg, ATGP0522-500 µg, ATGP0522-1 mg, ATGP0522-10, ATGP0522-20, ATGP0522-50, ATGP0522-100, ATGP0522-250, ATGP0522-500, ATGP0522-1

References & Citations

Wu K., et al. (1997) Arch Biochem Biophys. 347 (2) :221-8. ; ; Jaiswal AK., et al. (1994) J Biol Chem. 269 (20) :14502-8. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Redox Proteins

NCBI Accession Number

AAH06096

Uniprot Accession Number

P16083

Species

Human

AA Sequence

MAGKKVLIVY AHQEPKSFNG SLKNVAVDEL SRQGCTVTVS DLYAMNFEPR ATDKDITGTL SNPEVFNYGV ETHEAYKQRS LASDITDEQK KVREADLVIF QFPLYWFSVP AILKGWMDRV LCQGFAFDIP GFYDSGLLQG KLALLSVTTG GTAEMYTKTG VNGDSRYFLW PLQHGTLHFC GFKVLAPQIS FAPEIASEEE RKGMVAAWSQ RLQTIWKEEP IPCTAHWHFG Q