Recombinant human Apolipoprotein M/APOM protein

Apolipoproteins are protein components of plasma lipoproteins. APOM is a member of the lipocalin family of proteins. APOM is exclusively expressed in kidney tubular epithelial cells and liver hepatocytes. APOM is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions. The concentration of APOM in plasma strongly correlates with total cholesterol. Low concentrations of APOM in plasma are associated with diabetes. Recombinant human APOM protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Apolipoprotein M, G3a, HSPC336, NG20, Apolipoprotein M Apo M, ApoM, G3A, MGC22400, NG20 like protein, Protein G3a.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.9 kDa (187aa) confirmed by MALDI-TOF

Additionnal Information

Apolipoprotein M, ApoM, Apolipoprotein M, G3a, HSPC336, NG20, Apolipoprotein M Apo M, ApoM, G3A, MGC22400, NG20 like protein, Protein G3a., ATGP0521-10 µg, ATGP0521-20 µg, ATGP0521-50 µg, ATGP0521-100 µg, ATGP0521-250 µg, ATGP0521-500 µg, ATGP0521-1 mg, ATGP0521-10, ATGP0521-20, ATGP0521-50, ATGP0521-100, ATGP0521-250, ATGP0521-500, ATGP0521-1

References & Citations

Jiang J., et al. (2008) Lipids Health Dis. 7:25. ; ; Duan J., et al. (2001) FEBS Lett. 499 (1-2) :127-32. ;

Other References

Takahashi C, et al. Vehicle-dependent Effects of Sphingosine 1-phosphate on Plasminogen Activator Inhibitor-1 Expression. (J Atheroscler Thromb. 2017) {https://pubmed.ncbi.nlm.nih.gov/28321011/}; ; Kurano M, et al. Induction of insulin secretion by apolipoprotein M, a carrier for sphingosine 1-phosphate. (Biochim Biophys Acta. 2014) {https://pubmed.ncbi.nlm.nih.gov/24814049/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Adhesion & Movement

NCBI Accession Number

NP_061974

Uniprot Accession Number

O95445

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MCPEHSQLTT LGVDGKEFPE VHLGQWYFIA GAAPTKEELA TFDPVDNIVF NMAAGSAPMQ LHLRATIRMK DGLCVPRKWI YHLTEGSTDL RTEGRPDMKT ELFSSSCPGG IMLNETGQGY QRFLLYNRSP HPPEKCVEEF KSLTSCLDSK AFLLTPRNQE ACELSNN