Recombinant human CCL27/CTACK protein
Cutaneous T cell-attracting chemokine CCL27 is a member of the chemokine superfamily and the subfamily of beta r C-C chemokines that binds chemokine receptor CCR10. Chemokines are a superfamily of small secreted proteins that attract their targets by interacting with G protein-coupled receptors expressed on the migrating cell. CCL27 is involved in the preferential migration and recruitment of cutaneous lymphocyte-associated antigen CLA+ memory lymphocytes. Recombinant human CCL27 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
10.2 kDa (89aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Homey B., et al. (2002) Nat Med. 8 (2) :157-65. ; ; Kanda N., et al. (2007) Clin Exp Allergy. 37 (7) :1074-82.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 10 mM Sodium Citrate buffer (pH 3.5) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_006655.1
Uniprot Accession Number
Q9Y4X3
Species
Human
AA Sequence
MFLLPPSTAC CTQLYRKPLS DKLLRKVIQV ELQEADGDCH LQAFVLHLAQ RSICIHPQNP SLSQWFEHQE RKLHGTLPKL NFGMLRKMG
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