Recombinant HCV NS3 protein
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
NS3 protease/helicase, NS3 protease/helicase HCV NS3 transactivated protein, NS 3, NS3P, P70, Serine protease/NTPase/helicase.
Expression System
E.coli
Antigen Species
HCV
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
27.9 kDa (268aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hahn JA., et al. (2007) J Infect Dis. 195 (11) :1556-9. ; ; Caruntu FA., et al. (2006) J Gastrointestin Live Dis. 15 (3) :249-56.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Virus
NCBI Accession Number
NP_671491
Species
HCV
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
