Recombinant human LTA/TNF-beta protein

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Recombinant TNF beta protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human TNF-beta induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Product Name Alternative

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, TNFB, TNFSF1, TNF superfamily, member 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 1 ng/mL.

Molecular Weight

20.9 kDa (192aa) confirmed by MALDI-TOF

Additionnal Information

Lymphotoxin a1, TNF b, Lymphotoxin alpha, CLTalpha, Coley's toxin, DIF, Differentiation inducing factor, Hemorrhagic factor, LT, LT alpha, LTA, LTalpha, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Natural killer cytotoxic factor, Necrosin, NKCF, OTTHuMP00000037612, OTTHuMP00000037613, TNF B, TNF superfamily member 1, TNFB, TNFbeta, TNFSF 1, TNFSF1, Tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1, Tumor necrosis factor superfamily member 1, Lymphotoxin alpha, ATGP0517-10 µg, ATGP0517-20 µg, ATGP0517-50 µg, ATGP0517-100 µg, ATGP0517-250 µg, ATGP0517-500 µg, ATGP0517-1 mg, ATGP0517-10, ATGP0517-20, ATGP0517-50, ATGP0517-100, ATGP0517-250, ATGP0517-500, ATGP0517-1

References & Citations

Messer G, et al. (1991) J Exp Med. 173 (1) :209-19; ; Banner DW, et al. (1993) Cell. 73 (3) :431-45.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001153212

Uniprot Accession Number

P01374

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL