Recombinant human H-Ras protein
HRAS, also known as GTPase HRas, belongs to the Ras family which function in signal transduction pathways. This protein can bind GTP and GDP, and it has intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. Defects in HRAS are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Recombinant human HRAS protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
22.0 kDa (194aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
McCormick F. (1995) Mol Reprod Dev. 42 (4) :500-6; ; Ayllon V. et al. (2000) Mol Membr Biol. 17 (2) :65-73;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001123914.1
Uniprot Accession Number
P01112
Species
Human
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
