Recombinant human Peroxiredoxin 4/PRDX4 protein

PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Product Name Alternative

Antioxidant enzyme AOE372, AOE37-2, Peroxiredoxin IV, Prx-IV, Thioredoxin peroxidase AO372, Thioredoxin-dependent peroxide reductase A0372, Thioredoxin-dependent peroxiredoxin 4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >2,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Molecular Weight

28.8 kDa (255aa) confirmed by MALDI-TOF

Additionnal Information

Peroxiredoxin4, PRDX4, ATGP0513-10 µg, ATGP0513-20 µg, ATGP0513-50 µg, ATGP0513-100 µg, ATGP0513-250 µg, ATGP0513-500 µg, ATGP0513-1 mg, ATGP0513-10, ATGP0513-20, ATGP0513-50, ATGP0513-100, ATGP0513-250, ATGP0513-500, ATGP0513-1

References & Citations

Jin D.-Y., et al. (1997) J. Biol. Chem. 272:30952-30961.; ; Wang HQ., et al. (2009) FEBS Lett. 583 (9) :1511-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_006397

Uniprot Accession Number

Q13162

Species

Human

AA Sequence

MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN