Recombinant human SOD2/Mn-SOD protein

SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant human SOD2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Product Name Alternative

Superoxide dismutase [Mn] mitochondrial, IPOB, MNSOD, MVCD6, Superoxide dismutase [Mn], mitochondrial IPO B, Manganese SOD, Manganese superoxide dismutase, Mn SOD, SOD 2, SOD2, Superoxide dismutase [Mn] mitochondrial, Superoxide dismutase 2 mitochondrial, Superoxide Dismutase-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,000unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Molecular Weight

24.4 kDa (219aa) confirmed by MALDI-TOF

Additionnal Information

SOD2, Mn-SOD, Superoxide dismutase [Mn] mitochondrial, IPOB, MNSOD, MVCD6, Superoxide dismutase [Mn], mitochondrial IPO B, Manganese SOD, Manganese superoxide dismutase, Mn SOD, SOD 2, SOD2, Superoxide dismutase [Mn] mitochondrial, Superoxide dismutase 2 mitochondrial, Superoxide Dismutase-2, ATGP0512-10 µg, ATGP0512-20 µg, ATGP0512-50 µg, ATGP0512-100 µg, ATGP0512-250 µg, ATGP0512-500 µg, ATGP0512-1 mg, ATGP0512-10, ATGP0512-20, ATGP0512-50, ATGP0512-100, ATGP0512-250, ATGP0512-500, ATGP0512-1

References & Citations

MacMillan-Crow L.A., et al. (1999) Arch. Biochem. Biophys. 366:82-88; ; Prunotto M, et al. (2010) J Am Soc Nephrol. 21 (3) :507-19.;

Other References

Kwon MJ, et al. Superoxide dismutase 3 suppresses hyaluronic acid fragments mediated skin inflammation by inhibition of toll-like receptor 4 signaling pathway: superoxide dismutase 3 inhibits reactive oxygen species-induced trafficking of toll-like receptor 4 to lipid rafts. (Antioxid Redox Signal. 2012) {https://pubmed.ncbi.nlm.nih.gov/21957979/}; ; Kwon MJ, et al. Superoxide dismutase 3 controls adaptive immune responses and contributes to the inhibition of ovalbumin-induced allergic airway inflammation in mice. (Antioxid Redox Signal. 2012) {https://pubmed.ncbi.nlm.nih.gov/22583151/}; ; Peskin AV, Winterbourn CC. Assay of superoxide dismutase activity in a plate assay using WST-1. (Free Radic Biol Med. 2017) {https://pubmed.ncbi.nlm.nih.gov/28017897/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_001019636.1

Uniprot Accession Number

P04179

Species

Human

AA Sequence

KHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK