Recombinant human HAT1 protein

HAT1, also known as histone acetyltransferase 1, is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Recombinant HAT1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Histone acetyltransferase 1, KAT1, Histone acetyltransferase 1 HAT 1, Histidine aminotransferase 1, Histone acetyltransferase type B catalytic subunit

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

40 kDa (343aa) confirmed by MALDI-TOF

Additionnal Information

HAT1, Histone acetyltransferase 1, KAT1, Histone acetyltransferase 1 HAT 1, Histidine aminotransferase 1, Histone acetyltransferase type B catalytic subunit, ATGP0507-10 µg, ATGP0507-20 µg, ATGP0507-50 µg, ATGP0507-100 µg, ATGP0507-250 µg, ATGP0507-500 µg, ATGP0507-1 mg, ATGP0507-10, ATGP0507-20, ATGP0507-50, ATGP0507-100, ATGP0507-250, ATGP0507-500, ATGP0507-1

References & Citations

Verreault A., et al. (1998) Curr Biol. 8 (2) :96-108. ; ; Marmorstein R., et al. (2001) J Mol Biol. 311 (3) :433-44. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_003633

Uniprot Accession Number

O14929

Species

Human

AA Sequence

KKLAEYKCN TNTAIELKLV RFPEDLENDI RTFFPEYTHQ LFGDDETAFG YKGLKILLYY IAGSLSTMFR VEYASKVDEN FDCVEADDVE GKIRQIIPPG FCTNTNDFLS LLEKEVDFKP FGTLLHTYSV LSPTGGENFT FQIYKADMTC RGFREYHERL QTFLMWFIET ASFIDVDDER WHYFLVFEKY NKDGATLFAT VGYMTVYNYY VYPDKTRPRV SQMLILTPFQ GQGHGAQLLE TVHRYYTEFP TVLDITAEDP SKSYVKLRDF VLVKLCQDLP CFSREKLMQG FNEDMAIEAQ QKFKINKQHA RRVYEILRLL VTD