Recombinant human Carbonic Anhydrase 3/CA3 protein

CA3, also known as carbonic anhydrase III, is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O = HCO3 + H+) . This protein is involved in a variety of biological processes, including respiration, calcifica-tion, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. It contains a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Recombinant CA3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Carbonic anhydrase III, Carbonate dehydratase III, Car3, CAIII, CA3

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.5 kDa (260aa) confirmed by MALDI-TOF

Additionnal Information

Carbonic Anhydrase III, CA3, Carbonic anhydrase III, CAIII, Car3, Carbonic anhydrase III, CA3, ATGP0503-10 µg, ATGP0503-20 µg, ATGP0503-50 µg, ATGP0503-100 µg, ATGP0503-250 µg, ATGP0503-500 µg, ATGP0503-1 mg, ATGP0503-10, ATGP0503-20, ATGP0503-50, ATGP0503-100, ATGP0503-250, ATGP0503-500, ATGP0503-1

References & Citations

Lindskog S., et al (1997) Pharmacol Ther, 74 (1) :1-20. ; ; Sawaya MR., et al (2006) J Biol Chem. 281 (11) :7546-55;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH04897

Uniprot Accession Number

P07451

Species

Human

AA Sequence

MAKEWGYASH NGPDHWHELF PNAKGENQSP IELHTKDIRH DPSLQPWSVS YDGGSAKTIL NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL VHWNPKYNTF KEALKQRDGI AVIGIFLKIG HENGEFQIFL DALDKIKTKG KEAPFTKFDP SCLFPACRDY WTYQGSFTTP PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL VSNWRPPQPI NNRVVRASFK