Recombinant human HADH protein
HADH, which belongs to the family of oxidoreductases, is important for converting certain fats to energy. This protein is an enzyme that catalyzes the chemical reaction. ((S) -3-hydroxyacyl-CoA + NAD+ <=>3-oxoacyl-CoA + NADH + H+) It is also involved in a process called fatty acid oxidation, in which several enzymes work in a step-wise fashion to break down (metabolize) fats and convert them to energy. Recombinant HADH protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
35.1 kDa (323aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Tieu K, et al. (2004) Ann Neurol. 56 (1) :51-60; ; Vredendaal PJ, et al. (1996) Biochem Biophys Res Commun. 223 (3) :718-23.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
AAH00306
Uniprot Accession Number
Q16836
Species
Human
AA Sequence
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