Recombinant human Cyclophilin-like 2/PPIL2 protein

PPIL2, which belongs to the cyclophilin family of peptidylprolyl isomerases, is an enzyme that plays an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Recombinant PPIL2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 290 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin..

Product Name Alternative

UBOX7, U-box domain containing 7, RING-type E3 ubiquitin-protein ligase PPIL2, PPIase, Peptidylprolyl isomerase like 2, Peptidyl-prolyl cis-trans isomerase-like 2, hCyP-60, Cyp60, Cyclophilin-like protein Cyp-60, Cyclophilin-60, CYC4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 290 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin..

Molecular Weight

61.6 kDa (547aa)

Additionnal Information

PPIL2, Peptidyl-prolyl cis-trans isomerase-like 2, CYC4, Cyp-60, CYP60, hCyP-60, GC33174, Peptidyl-prolyl cis-trans isomerase-like 2 Cyclophilin 60, Cyclophilin like protein Cyp 60, CYP60, Peptidyl prolyl cis trans isomerase like 2, PPIase, Rotamase PPIL2., ATGP0498-10 µg, ATGP0498-20 µg, ATGP0498-50 µg, ATGP0498-100 µg, ATGP0498-250 µg, ATGP0498-500 µg, ATGP0498-1 mg, ATGP0498-10, ATGP0498-20, ATGP0498-50, ATGP0498-100, ATGP0498-250, ATGP0498-500, ATGP0498-1

References & Citations

Wang BB, et al. (1996) Biochem J. 314:313-9.; ; Carson R, et al. (2009) Neuromolecular Med. 11 (4) :337-44.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_680481.1

Uniprot Accession Number

Q13356

Species

Human

AA Sequence

MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF TSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGS SRLPDDH