Recombinant human Cornulin/CRNN protein

CRNN is a member of the 'fused gene' family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. It contains two EF-hand Ca2+ binding domains in its N-terminus and two glutamine- and threonine-rich 60 amino acid repeats in its C-terminus. This protein, also Known as Survival factor that participates in the clonogenicity of squamous esophageal epithelium cell lines, attenuates deoxycholic acid (DCA) -induced apoptotic cell death and release of calcium. When overexpressed in oral squamous carcinom cell lines, regulates negatively cell proliferation by the induction of G1 arrest. Recombinant CRNN protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cornulin, DRC1, PDRC1, SEP53, Cornulin 53 kDa putative calcium binding protein, 53 kDa squamous epithelial induced stress protein, 58 kDa heat shock protein, C1orf10, Squamous epithelial heat shock protein 53, Tumor related protein.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

55.7 kDa (515aa)

Additionnal Information

Cornulin, CRNN, Cornulin, DRC1, PDRC1, SEP53, Cornulin 53 kDa putative calcium binding protein, 53 kDa squamous epithelial induced stress protein, 58 kDa heat shock protein, C1orf10, Squamous epithelial heat shock protein 53, Tumor related protein., ATGP0497-10 µg, ATGP0497-20 µg, ATGP0497-50 µg, ATGP0497-100 µg, ATGP0497-250 µg, ATGP0497-500 µg, ATGP0497-1 mg, ATGP0497-10, ATGP0497-20, ATGP0497-50, ATGP0497-100, ATGP0497-250, ATGP0497-500, ATGP0497-1

References & Citations

Little TJ., et al. (2007) PLoS One. 2 (10) :e1003.; ; Darragh J., et al. (2006) FEBS J. 273:1930-1947;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Adhesion & Movement

NCBI Accession Number

AAH30807

Uniprot Accession Number

Q9UBG3

Species

Human

AA Sequence

MPQLLQNING IIEAFRRYAR TEGNCTALTR GELKRLLEQE FADVIVKPHD PATVDEVLRL LDEDHTGTVE FKEFLVLVFK VAQACFKTLS ESAEGACGSQ ESGSLHSGAS QELGEGQRSG TEVGRAGKGQ HYEGSSHRQS QQGSRGQNRP GVQTQGQATG SAWVSSYDRQ AESQSQERIS PQIQLSGQTE QTQKAGEGKR NQTTEMRPER QPQTREQDRA HQTGETVTGS GTQTQAGATQ TVEQDSSHQT GRTSKQTQEA TNDQNRGTET HGQGRSQTSQ AVTGGHAQIQ AGTHTQTPTQ TVEQDSSHQT GSTSTQTQES TNGQNRGTEI HGQGRSQTSQ AVTGGHTQIQ AGSHTETVEQ DRSQTVSHGG AREQGQTQTQ PGSGQRWMQV SNPEAGETVP GGQAQTGAST EPGRQEWSST HPRRCVTEGQ GDRQPTVVGE EWVDDHSRET VILRLDQGNL HTSVSSAQGQ DAAQSEEKRG ITARELYSYL RSTKP