Recombinant human HSPB8 protein

Heat shock protein beta-8 (HSPB8), also known as HSP22, member of the small heat shock protein superfamily. HSPB8 is predominantly transcribed in skeletal muscle and heart. The chaperone-like activity is of great importance to the function of HSPB8 in various processes including proliferation, apoptosis and macroautophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) . Recombinant human HSPB8, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heat shock protein beta-8, CRYAC, E2IG1, CMT2L, DHMN2, HMN2, HMN2A, Heat shock protein beta-8, heat shock 22kDa protein 8, H11, HSP22, HspB8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.7 kDa (216aa) confirmed by MALDI-TOF

Additionnal Information

HSPB8, Heat shock protein beta-8, CRYAC, E2IG1, CMT2L, DHMN2, HMN2, HMN2A, Heat shock protein beta-8, heat shock 22kDa protein 8, H11, HSP22, HspB8, ATGP0493-10 µg, ATGP0493-20 µg, ATGP0493-50 µg, ATGP0493-100 µg, ATGP0493-250 µg, ATGP0493-500 µg, ATGP0493-1 mg, ATGP0493-10, ATGP0493-20, ATGP0493-50, ATGP0493-100, ATGP0493-250, ATGP0493-500, ATGP0493-1

References & Citations

Sun X., et al. (2004) J Biol Chem. 279 (4) :2394-402. ; ; Benndorf R., et al. (2001) J Biol Chem. 276:26753-61. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 20% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_055180.1

Uniprot Accession Number

Q9UJY1

Species

Human

AA Sequence

MADGQMPFSC HYPSRLRRDP FRDSPLSSRL LDDGFGMDPF PDDLTASWPD WALPRLSSAW PGTLRSGMVP RGPTATARFG VPAEGRTPPP FPGEPWKVCV NVHSFKPEEL MVKTKDGYVE VSGKHEEKQQ EGGIVSKNFT KKIQLPAEVD PVTVFASLSP EGLLIIEAPQ VPPYSTFGES SFNNELPQDS QEVTCT