Recombinant human N-RAS protein

Neuroblastoma RAS viral (v-ras) oncogene homolog, also known as NRAS, is a member of the RAS gene family. NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Recombinant human NRAS was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GTPase Nras, NRAS proto-oncogene GTPase, Neuroblastoma RAS viral (v-ras) oncogene homolog, neuroblastoma RAS viral oncogene homolog, Transforming protein N-Ras, N-ras, NRAS, HRAS1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

20.8 kDa (186aa) confirmed by MALDI-TOF

Additionnal Information

NRAS, Neuroblastoma RAS viral (v-ras) oncogene homolog, GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, AV095280, N ras, N ras protein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHuMP00000013879, Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog, ATGP0492-10 µg, ATGP0492-20 µg, ATGP0492-50 µg, ATGP0492-100 µg, ATGP0492-250 µg, ATGP0492-500 µg, ATGP0492-1 mg, ATGP0492-10, ATGP0492-20, ATGP0492-50, ATGP0492-100, ATGP0492-250, ATGP0492-500, ATGP0492-1

References & Citations

Brown R., et al. (1984) EMBO J. 3 (6) :1321-6. ; ; McCormick., et al. (1995) Mol Reprod Dev. 42 (4) :500-6.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_002515

Uniprot Accession Number

P01111

Species

Human

AA Sequence

MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV REIRQYRMKK LNSSDDGTQG CMGLPC