Recombinant human MAT1A protein

MAT1A catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver. MAT1A expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT2A present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NFkappaB and TNFalpha cause a switch from MAT1A to MAT2A expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth. Recombinant human MAT1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

S-adenosylmethionine synthetase, MAT, MATA1, SAMS, SAMS1, methionine adenosyltransferase I alpha, S-adenosylmethionine synthetase, MAT1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

45.8 kDa (415aa) confirmed by MALDI-TOF

Additionnal Information

MAT1A, S-adenosylmethionine synthetase, MAT, MATA1, SAMS, SAMS1, methionine adenosyltransferase I alpha, S-adenosylmethionine synthetase, MAT1A, ATGP0489-10 µg, ATGP0489-20 µg, ATGP0489-50 µg, ATGP0489-100 µg, ATGP0489-250 µg, ATGP0489-500 µg, ATGP0489-1 mg, ATGP0489-10, ATGP0489-20, ATGP0489-50, ATGP0489-100, ATGP0489-250, ATGP0489-500, ATGP0489-1

References & Citations

Ding W., et al. (2009) Hepatology. 49 (4) :1277-86. ; ; Tomasi ML., et al. (2009) Gastroenterology. 136 (3) :1025-36.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000420.1

Uniprot Accession Number

Q00266

Species

Human

AA Sequence

MNGPVDGLCD HSLSEGVFMF TSESVGEGHP DKICDQISDA VLDAHLKQDP NAKVACETVC KTGMVLLCGE ITSMAMVDYQ RVVRDTIKHI GYDDSAKGFD FKTCNVLVAL EQQSPDIAQC VHLDRNEEDV GAGDQGLMFG YATDETEECM PLTIILAHKL NARMADLRRS GLLPWLRPDS KTQVTVQYMQ DNGAVIPVRI HTIVISVQHN EDITLEEMRR ALKEQVIRAV VPAKYLDEDT VYHLQPSGRF VIGGPQGDAG VTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW VAKSLVKAGL CRRVLVQVSY AIGVAEPLSI SIFTYGTSQK TERELLDVVH KNFDLRPGVI VRDLDLKKPI YQKTACYGHF GRSEFPWEVP RKLVF