Recombinant human Lactate Dehydrogenase A/LDHA protein

LDHA, also known as L-lactate dehydrogenase A chain, is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. Recombinant LDHA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Product Name Alternative

Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 300unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Molecular Weight

38.8 kDa (352aa) confirmed by MALDI-TOF

Additionnal Information

Lactate Dehydrogenase A, LDHA, L-lactate dehydrogenase A chain, GSD11, LDH1, LDHM, PIG19, L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein, LDH muscle subunit, M LDH, L lactate dehydrogenase A chain, l7R2, Lactate dehydrogenase 1, A chain, Lactate dehydrogenase A, Lactate dehydrogenase A4, Lactate dehydrogenase M, LDH muscle subunit, OTTMuSP00000017774, Proliferation-inducing gene 19, Renal carcinoma antigen NY-REN-59., ATGP0488-10 µg, ATGP0488-20 µg, ATGP0488-50 µg, ATGP0488-100 µg, ATGP0488-250 µg, ATGP0488-500 µg, ATGP0488-1 mg, ATGP0488-10, ATGP0488-20, ATGP0488-50, ATGP0488-100, ATGP0488-250, ATGP0488-500, ATGP0488-1

References & Citations

Gosti F, et al. (1987) Proc. Natl. Acad. Sci. u.S.A. 84 (4) :1000-4; ; Chung FZ, et al. (1985) Biochem. J. 231 (3) :537-41.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_005557

Uniprot Accession Number

P00338

Species

Human

AA Sequence

MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF