Recombinant human IVD protein

IVD (Isovaleryl Coenzyme A dehydrogenase) is a mitochondrial matrix enzyme that belongs to the acyl-CoA dehydrogenase family. IVD is a homotetrameric flavoenzyme which catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA. Defects of the IVD gene lead to ineffective isoforms that are the underlying cause of isovaleric acidemia. Recombinant human IVD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Isovaleryl-CoA dehydrogenase mitochondrial, ACAD2, Isovaleryl-CoA dehydrogenase, mitochondrial Isovaleryl CoA dehydrogenase, Isovaleryl CoA dehydrogenase, mitochondrial, isovaleryl Coenzyme A dehydrogenase.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.3 kDa (415aa) confirmed by MALDI-TOF

Additionnal Information

IVD, ATGP0487-10 µg, ATGP0487-20 µg, ATGP0487-50 µg, ATGP0487-100 µg, ATGP0487-250 µg, ATGP0487-500 µg, ATGP0487-1 mg, ATGP0487-10, ATGP0487-20, ATGP0487-50, ATGP0487-100, ATGP0487-250, ATGP0487-500, ATGP0487-1

References & Citations

Hodges PW., et al. (2009) Eur J Neurosci. 29 (7) :1490-500. ; ; Rohini R., et al. (2009) Eur J Med Chem. 44 (8) :3330-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_002216.2

Uniprot Accession Number

P26440

Species

Human

AA Sequence

MHSLLPVDDA INGLSEEQRQ LRQTMAKFLQ EHLAPKAQEI DRSNEFKNLR EFWKQLGNLG VLGITAPVQY GGSGLGYLEH VLVMEEISRA SGAVGLSYGA HSNLCINQLV RNGNEAQKEK YLPKLISGEY IGALAMSEPN AGSDVVSMKL KAEKKGNHYI LNGNKFWITN GPDADVLIVY AKTDLAAVPA SRGITAFIVE KGMPGFSTSK KLDKLGMRGS NTCELIFEDC KIPAANILGH ENKGVYVLMS GLDLERLVLA GGPLGLMQAV LDHTIPYLHV REAFGQKIGH FQLMQGKMAD MYTRLMACRQ YVYNVAKACD EGHCTAKDCA GVILYSAECA TQVALDGIQC FGGNGYINDF PMGRFLRDAK LYEIGAGTSE VRRLVIGRAF NADFH