Recombinant human GOPC protein

GOPC, also known as PIST, is a PDZ domain-containing Golgi protein. This protein functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, GOPC is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding GOPC are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis. Recombinant GOPC protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Golgi-associated PDZ and coiled-coil motif-containing protein, CAL, dJ94G16.2, FIG, GOPC1, PIST, Golgi-associated PDZ and coiled-coil motif-containing protein CFTR associated ligand, dJ94G16.2, FIG Fused in glioblastoma, Golgi associated PDZ and coiled coil motif containing, Golgi associated PDZ and coiled coil motif containing protein, GOPC 1, OTTHuMP00000040403, PDZ protein interacting specifically with TC 10, PDZ protein interacting specifically with TC10, PDZ/coiled coil domain binding partner for the rho family GTPase TC 10, PDZ/coiled coil domain binding partner for the rho family GTPase TC10, Protein interacting specifically with Tc 10, Protein interacting specifically with Tc10.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.5 kDa (198aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

GOPC, Golgi-associated PDZ and coiled-coil motif-containing protein, CAL, dJ94G16.2, FIG, GOPC1, PIST, Golgi-associated PDZ and coiled-coil motif-containing protein CFTR associated ligand, dJ94G16.2, FIG Fused in glioblastoma, Golgi associated PDZ and coiled coil motif containing, Golgi associated PDZ and coiled coil motif containing protein, GOPC 1, OTTHuMP00000040403, PDZ protein interacting specifically with TC 10, PDZ protein interacting specifically with TC10, PDZ/coiled coil domain binding partner for the rho family GTPase TC 10, PDZ/coiled coil domain binding partner for the rho family GTPase TC10, Protein interacting specifically with Tc 10, Protein interacting specifically with Tc10., ATGP0485-10 µg, ATGP0485-20 µg, ATGP0485-50 µg, ATGP0485-100 µg, ATGP0485-250 µg, ATGP0485-500 µg, ATGP0485-1 mg, ATGP0485-10, ATGP0485-20, ATGP0485-50, ATGP0485-100, ATGP0485-250, ATGP0485-500, ATGP0485-1

References & Citations

Cheng J., et al. (2002) J Biol Chem. 277 (5) :3520-9. ; ; Yao R., et al. (2001) Biochem Biophys Res Commun. 286 (4) :771-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Adhesion & Movement

NCBI Accession Number

NP_001017408

Uniprot Accession Number

Q9HD26

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MIRKVLLLKE DHEGLGISIT GGKEHGVPIL ISEIHPGQPA DRCGGLHVGD AILAVNGVNL RDTKHKEAVT ILSQQRGEIE FEVVYVAPEV DSDDENVEYE DESGHRYRLY LDELEGGGNP GASCKDTSGE IKVLQGFNKK AVTDTHENGD LGTASETPLD DGASKLDDLH TLYHKKSY