Recombinant human APRT protein

APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Adenine phosphoribosyltransferase, AMP, Adenine phosphoribosyltransferase AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, MGC125856, MGC125857, MGC129961, Transphosphoribosidase.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.6 kDa (180aa) confirmed by MALDI-TOF

Additionnal Information

APRT, Adenine phosphoribosyltransferase, AMP, Adenine phosphoribosyltransferase AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, MGC125856, MGC125857, MGC129961, Transphosphoribosidase., ATGP0483-10 µg, ATGP0483-20 µg, ATGP0483-50 µg, ATGP0483-100 µg, ATGP0483-250 µg, ATGP0483-500 µg, ATGP0483-1 mg, ATGP0483-10, ATGP0483-20, ATGP0483-50, ATGP0483-100, ATGP0483-250, ATGP0483-500, ATGP0483-1

References & Citations

Baranowska-Bosiacka I., et al. (2009) Toxicology. 259 (1-2) :77-83.; ; Liang L., et al. (2007) Cancer Res. 67 (5) :1910-7.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000476

Uniprot Accession Number

P07741

Species

Human

AA Sequence

MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE