Recombinant human AprataxiPTX protein

Aprataxin is a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. Aprataxin may play a role in single-stranded DNA repair. Mutations in the gene have been associated with neurological disorder known as ataxia oculomotor apraxia-1. Recombinant Aprataxin protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Aprataxin, AXA1, Ataxia 1 early onset with hypoalbuminemia, AOA, AOA1, EAOH, EOAHA, Forkhead-associated domain histidine triad-like protein, FHA-HIT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.9 kDa (205aa) confirmed by MALDI-TOF

Additionnal Information

AOA, AOA 1, AOA1, AOA-1, Aprataxin, Aprataxin homolog, APTX, Ataxia 1 early onset with hypoalbuminemia, Ataxia1 early onset with hypoalbuminemia, AXA 1, AXA1, AXA-1, EAOH, EOAHA, FHA HIT, FHA-HIT, FLJ20157, Forkhead associated domain histidine triad like, Forkhead associated domain histidine triad like protein, Forkhead-associated domain histidine triad-like protein, MGC1072, ATGP0479-10 µg, ATGP0479-20 µg, ATGP0479-50 µg, ATGP0479-100 µg, ATGP0479-250 µg, ATGP0479-500 µg, ATGP0479-1 mg, ATGP0479-010, ATGP0479-020, ATGP0479-050, ATGP0479-100, ATGP0479-250, ATGP0479-500, ATGP0479-01M

References & Citations

Kijas A.W., et al (2006) J. Biol. Chem. 281:13939-13948; ; Rass u., et al. (2007) J. Biol. Chem. 282:9469-9474

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 100 mM NaCl, 0.1mM PMSF

Scientific Category

Neuroscience

NCBI Accession Number

NP_778241

Uniprot Accession Number

Q7Z2E3

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMQD PKMQVYKDEQ VVVIKDKYPK ARYHWLVLPW TSISSLKAVA REHLELLKHM HTVGEKVIVD FAGSSKLRFR LGYHAIPSMS HVHLHVISQD FDSPCLKNKK HWNSFNTEYF LESQAVIEMV QEAGRVTVRD GMPELLKLPL RCHECQQLLP SIPQLKEHLR KHWTQ