Recombinant human Aldo-keto reductase 7A2/AKR7A2 protein
Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to gamma-Hydroxybutyrate in human brain. Recombinant AKR7A2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
44 kDa (398aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bodreddigari S., et al. (2008) Chem Res Toxicol. 21 (5) :1134-42.; ; Malaspina P., et al. (2009) Hum Genomics. 3 (2) :106-20.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_003680
Uniprot Accession Number
O43488
Species
Human
AA Sequence
Frequently Asked Questions
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