Recombinant human Aldolase A/ALDOA protein

Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1, 6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 10 units/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3-phosphate per minute at pH 7.5 at 37C.

Product Name Alternative

Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 10unit/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3-phosphate per minute at pH 7.5 at 37C.

Molecular Weight

41.5 kDa (384aa) confirmed by MALDI-TOF

Additionnal Information

Aldolase A, Fructose bisphosphate aldolase A, ALDOA, ALDA, GSD12, Fructose bisphosphate aldolase A Aldolase A, Aldolase A fructose bisphosphatase, Aldolase A fructose bisphosphate, Fructose 1 6 bisphosphate triosephosphate lyase, Fructose bisphosphate aldolase, Lung cancer antigen NY Lu 1, MGC10942, MGC17716, MGC17767, Muscle type aldolase, ATGP0477-10 µg, ATGP0477-20 µg, ATGP0477-50 µg, ATGP0477-100 µg, ATGP0477-250 µg, ATGP0477-500 µg, ATGP0477-1 mg, ATGP0477-10, ATGP0477-20, ATGP0477-50, ATGP0477-100, ATGP0477-250, ATGP0477-500, ATGP0477-1

References & Citations

Pfleiderer G., et al. (1975) Beitr Pathol. 156 (3) :266-79. ; ; Kishi H., et al. (1987) Proc Natl Acad uSA. 84 (23) :8623-7.

Other References

Jung SH, et al. Integrative Proteomic Profiling of Protein Activity and Interactions Using Protein Arrays. (Mol Cell Proteomics. 2012) {https://www.ncbi.nlm.nih.gov/pubmed/22843993}; ; Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27040940/}; ; Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017) {https://pubmed.ncbi.nlm.nih.gov/28536714/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_908930

Uniprot Accession Number

P04075

Species

Human

AA Sequence

MPYQYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS ALAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HIYLEGTLLK PNMVTPGHAC TQKFSHEEIA MATVTALRRT VPPAVTGITF LSGGQSEEEA SINLNAINKC PLLKPWALTF SYGRALQASA LKAWGGKKEN LKAAQEEYVK RALANSLACQ GKYTPSGQAG AAASESLFVS NHAY