Recombinant human Aldo-keto reductase 7A3/AKR7A3 protein

Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Product Name Alternative

Aldo-keto reductase family 7 member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2 (AFAR2), AFB1 AR 2, Aflatoxin aldehyde reductase, Aflatoxin B1 aldehyde reductase 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Molecular Weight

41.6 kDa (370aa) confirmed by MALDI-TOF

Additionnal Information

Aflatoxin B1 aldehyde reductase member3, AKR7A3, Aldo-keto reductase family 7 member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2 (AFAR2), Aldo-keto reductase family 7, member A 3 AFAR 2, AFAR2, AFB1 AR 2, Aflatoxin aldehyde reductase, Aflatoxin B1 aldehyde reductase 2, Aflatoxin B1 aldehyde reductase member 3, Aldo keto reductase family 7 member A3, OTTHuMP00000002623., ATGP0476-10 µg, ATGP0476-20 µg, ATGP0476-50 µg, ATGP0476-100 µg, ATGP0476-250 µg, ATGP0476-500 µg, ATGP0476-1 mg, ATGP0476-10, ATGP0476-20, ATGP0476-50, ATGP0476-100, ATGP0476-250, ATGP0476-500, ATGP0476-1

References & Citations

Kniqht LP., et al. (1999) Carcinogenesis. 20 (7) :1215-23. ; ; Bodreddiqari S., et al. (2008) Chem Res Toxicol. 21 (5) :1134-42.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

AAH25709.1

Uniprot Accession Number

O95154

Species

Human

AA Sequence

M SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR