Recombinant human HEXIM1 protein

HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This protein has been shown to form a protein-RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1, and to inhibit the kinase activity of CDK9, thereby suppressing RNA polymerase II-dependent transcriptional elongation. Recombinant HEXIM1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hexamethylene bis-acetamide inducible 1, CLP1, EDG1, HIS1, MAQ1, Hexamethylene bis-acetamide inducible 1 Cardiac lineage protein 1, CLP 1, EDG 1, FLJ13562, Hexamethylene bis acetamide inducible 1, Hexamethylene bis acetamide inducible protein, Hexamethylene bis acetamide inducible transcript 1, HEXIM 1, HEXIM1 protein, HIS 1, HMBA inducible, MAQ 1, Menage a quatre 1.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.8 kDa (396aa) confirmed by MALDI-TOF

Additionnal Information

HEXIM1, Hexamethylene bis-acetamide inducible 1, CLP1, EDG1, HIS1, MAQ1, Hexamethylene bis-acetamide inducible 1 Cardiac lineage protein 1, CLP 1, EDG 1, FLJ13562, Hexamethylene bis acetamide inducible 1, Hexamethylene bis acetamide inducible protein, Hexamethylene bis acetamide inducible transcript 1, HEXIM 1, HEXIM1 protein, HIS 1, HMBA inducible, MAQ 1, Menage a quatre 1., ATGP0475-10 µg, ATGP0475-20 µg, ATGP0475-50 µg, ATGP0475-100 µg, ATGP0475-250 µg, ATGP0475-500 µg, ATGP0475-1 mg, ATGP0475-10, ATGP0475-20, ATGP0475-50, ATGP0475-100, ATGP0475-250, ATGP0475-500, ATGP0475-1

References & Citations

Shimizu N., et al. (2005) Proc. Natl. Acad. Sci. u.S.A. 102 (24) :8555-60; ; Li Q., et al. (2007) Nucleic Acids Res 35 (8) :2503-12.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006451.1

Uniprot Accession Number

O94992

Species

Human

AA Sequence

MAE PFLSEYQHQP QTSNCTGAAA VQEELNPERP PGAEERVPEE DSRWQSRAFP QLGGRPGPEG EGSLESQPPP LQTQACPESS CLREGEKGQN GDDSSAGGDF PPPAEVEPTP EAELLAQPCH DSEASKLGAP AAGGEEEWGQ QQRQLGKKKH RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRIRAEM FAKGQPVAPY NTTQFLMDDH DQEEPDLKTG LYSKRAAAKS DDTSDDDFME EGGEEDGGSD GMGGDGSEFL QRDFSETYER YHTESLQNMS KQELIKEYLE LEKCLSRMED ENNRLRLESK RLGGDDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP LSKFGD