Recombinant human Aldehyde Dehydrogenase 3-A1/ALDH3A1 protein
ALDH3A1, also known as Aldehyde dehydrogenase 3 family memberA1, is involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to uV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. Recombinant ALDH3A1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
52.5 kDa (473aa)
Additionnal Information
References & Citations
Estey T., et al. (2007) Exp Eye Res. 84 (1) :3-12; ; Bogucka M, et al. (2009) Acta Pol Pharm. 66 (5) :477-82.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Scientific Category
Redox Proteins
NCBI Accession Number
AAH04370.1
Uniprot Accession Number
P30838
Species
Human
AA Sequence
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