Recombinant human PARP protein
PARP1 is a nuclear DNA-binding zinc finger protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP1 also plays a role in other cellular processes, including cell proliferation and differentiation. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly (ADPribosyl) ation contributes to the efficient maintenance of genome integrity. Recombinant PARP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
39.6 kDa (354aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Dantzer F., et al. (1998) Nucleic Acids Res. 26 (8) :1891-8. ; ; Li Y., et al. (2006) Mol Cell Endocrinol. 257-258:35-46.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
AAH37545.1
Uniprot Accession Number
P09874
Species
Human
AA Sequence
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