Recombinant human PARP protein

PARP1 is a nuclear DNA-binding zinc finger protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP1 also plays a role in other cellular processes, including cell proliferation and differentiation. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly (ADPribosyl) ation contributes to the efficient maintenance of genome integrity. Recombinant PARP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Poly (ADP-ribose) polymerase family member 1, ADPRT, ADPRT1, pADPRT, pADPRT-1, PARP, PARP-1, PPOL, Poly (ADP-ribose) polymerase family, member 1 ADP ribosyltransferase (NAD+, poly (ADP ribose) polymerase), ADPRT 1, msPARP, NAD (+) ADP ribosyltransferase 1, pADPRT 1, PARP 1, PARP1, Poly (ADP ribose) polymerase 1, poly (ADP ribose) synthetase, poly (ADP ribosyl) transferase, Poly[ADP ribose] synthetase 1, sPARP 1, sPARP1.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

39.6 kDa (354aa) confirmed by MALDI-TOF

Additionnal Information

PARP, Poly (ADP-ribose) polymerase family member 1, ADPRT, ADPRT1, pADPRT, pADPRT-1, PARP, PARP-1, PPOL, Poly (ADP-ribose) polymerase family, member 1 ADP ribosyltransferase (NAD+, poly (ADP ribose) polymerase), ADPRT 1, msPARP, NAD (+) ADP ribosyltransferase 1, pADPRT 1, PARP 1, PARP1, Poly (ADP ribose) polymerase 1, poly (ADP ribose) synthetase, poly (ADP ribosyl) transferase, Poly[ADP ribose] synthetase 1, sPARP 1, sPARP1., ATGP0469-10 µg, ATGP0469-20 µg, ATGP0469-50 µg, ATGP0469-100 µg, ATGP0469-250 µg, ATGP0469-500 µg, ATGP0469-1 mg, ATGP0469-10, ATGP0469-20, ATGP0469-50, ATGP0469-100, ATGP0469-250, ATGP0469-500, ATGP0469-1

References & Citations

Dantzer F., et al. (1998) Nucleic Acids Res. 26 (8) :1891-8. ; ; Li Y., et al. (2006) Mol Cell Endocrinol. 257-258:35-46.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

AAH37545.1

Uniprot Accession Number

P09874

Species

Human

AA Sequence

MKSKLPKPVQ DLIKMIFDVE SMKKAMVEYE IDLQKMPLGK LSKRQIQAAY SILSEVQQAV SQGSSDSQIL DLSNRFYTLI PHDFGMKKPP LLNNADSVQA KAEMLDNLLD IEVAYSLLRG GSDDSSKDPI DVNYEKLKTD IKVVDRDSEE AEIIRKYVKN THATTHNAYD LEVIDIFKIE REGECQRYKP FKQLHNRRLL WHGSRTTNFA GILSQGLRIA PPEAPVTGYM FGKGIYFADM VSKSANYCHT SQGDPIGLIL LGEVALGNMY ELKHASHISK LPKGKHSVKG LGKTTPDPSA NISLDGVDVP LGTGISSGVN DTSLLYNEYI VYDIAQVNLK YLLKLKFNFK TSLW