Recombinant human eIF-1A/EIF1 protein

EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sui1iso1, SuI1, Protein translation factor SUI1 homolog, ISO1, Eukaryotic translation initiation factor 1, EIF1A, EIF-1, EIF1, A121

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

16.9 kDa (150aa) confirmed by MALDI-TOF

Additionnal Information

Sui1iso1, SuI1, Protein translation factor SUI1 homolog, ISO1, Eukaryotic translation initiation factor 1, EIF1A, EIF-1, EIF1, A121, ATGP0461-10 µg, ATGP0461-20 µg, ATGP0461-50 µg, ATGP0461-100 µg, ATGP0461-250 µg, ATGP0461-500 µg, ATGP0461-1 mg, ATGP0461-010, ATGP0461-020, ATGP0461-050, ATGP0461-100, ATGP0461-250, ATGP0461-500, ATGP0461-01M

References & Citations

Dennis MD., et al. (2009) J Biol Chem. 284 (31) :20615-28. ; ; Pisarev AV., et al. (2007) Cell. 131 (2) :286-99.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005792

Uniprot Accession Number

P41567

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF