Recombinant human eIF-1A/EIF1 protein
EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Sui1iso1, SuI1, Protein translation factor SUI1 homolog, ISO1, Eukaryotic translation initiation factor 1, EIF1A, EIF-1, EIF1, A121
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
16.9 kDa (150aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Dennis MD., et al. (2009) J Biol Chem. 284 (31) :20615-28. ; ; Pisarev AV., et al. (2007) Cell. 131 (2) :286-99.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_005792
Uniprot Accession Number
P41567
Species
Human
AA Sequence
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