Recombinant human PRPS1 protein
PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.9 kDa (338aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Liu H., et al. (2009) Biochem Biophys Res Commun. 379 (4) :1120-5. ; ; Li S., et al. (2007) Biochem J. 401 (1) :39-47.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 20% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002755
Uniprot Accession Number
P60891
Species
Human
AA Sequence
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