Recombinant human PRPS1 protein

PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1, Ribose-phosphate pyrophosphokinase 1 EC 2.7.6.1, KIAA0967, Phosphoribosyl pyrophosphate synthetase I, PRS I, PRS-I, Ribose phosphate pyrophosphokinase I

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.9 kDa (338aa) confirmed by MALDI-TOF

Additionnal Information

PRPS1, Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1, Ribose-phosphate pyrophosphokinase 1 EC 2.7.6.1, KIAA0967, Phosphoribosyl pyrophosphate synthetase I, PRS I, PRS-I, Ribose phosphate pyrophosphokinase I, ATGP0460-10 µg, ATGP0460-20 µg, ATGP0460-50 µg, ATGP0460-100 µg, ATGP0460-250 µg, ATGP0460-500 µg, ATGP0460-1 mg, ATGP0460-10, ATGP0460-20, ATGP0460-50, ATGP0460-100, ATGP0460-250, ATGP0460-500, ATGP0460-1

References & Citations

Liu H., et al. (2009) Biochem Biophys Res Commun. 379 (4) :1120-5. ; ; Li S., et al. (2007) Biochem J. 401 (1) :39-47.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002755

Uniprot Accession Number

P60891

Species

Human

AA Sequence

MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI RRTHNGESVS YLFSHVPL