Recombinant human ENSA protein
ENSA, also known as alpha endosulfine, belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein is expressed in a wide range of tissues including muscle, brain, and endocrine tissues. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SuR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. Recombinant ENSA protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Alpha endosulfine isoform 3, ARPP-19e, Alpha endosulfine isoform 3 Alpha endosulfine, ARPP 19e, Endosulfine alpha.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15.5 kDa (141aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Heron L., et al. (1998) Proc Natl Acad Sci u S A. 95 (14) :8387-91. ; ; Kim SH., et al. (2001) J Neural Transm Suppl. 61:1-9. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.2mM PMSF
Scientific Category
Neuroscience
NCBI Accession Number
NP_004427
Uniprot Accession Number
O43768
Species
Human
AA Sequence
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