Recombinant human PTS protein

6-Pyruvoyltetrahydropterin synthase (PTS), also known as PTPS, belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH (4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. Recombinant human PTS, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

6-pyruvoyltetrahydropterin synthase, PTPS, PTP synthase, 6-pyruvoyltetrahydropterin synthase 6 pyruvoyl tetrahydrobiopterin synthase, 6 pyruvoyl tetrahydropterin synthase, 6 pyruvoyltetrahydropterin synthase, EC 4.2.3.12, FLJ97081

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.5 kDa (165aa) confirmed by MALDI-TOF

Additionnal Information

PTS, 6-pyruvoyltetrahydropterin synthase, PTPS, PTP synthase, 6-pyruvoyltetrahydropterin synthase 6 pyruvoyl tetrahydrobiopterin synthase, 6 pyruvoyl tetrahydropterin synthase, 6 pyruvoyltetrahydropterin synthase, EC 4.2.3.12, FLJ97081, ATGP0452-10 µg, ATGP0452-20 µg, ATGP0452-50 µg, ATGP0452-100 µg, ATGP0452-250 µg, ATGP0452-500 µg, ATGP0452-1 mg, ATGP0452-10, ATGP0452-20, ATGP0452-50, ATGP0452-100, ATGP0452-250, ATGP0452-500, ATGP0452-1

References & Citations

Thony B., et al. (1992) Biochem Biophys Res Commun. 189:1437-43. ; ; Milstien S., et al. (1989) J Biol Chem. 264 (14) :8066-73.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000308

Uniprot Accession Number

Q03393

Species

Human

AA Sequence

MSTEGGGRRC QAQVSRRISF SASHRLYSKF LSDEENLKLF GKCNNPNGHG HNYKVVVTVH GEIDPATGMV MNLADLKKYM EEAIMQPLDH KNLDMDVPYF ADVVSTTENV AVYIWDNLQK VLPVGVLYKV KVYETDNNIV VYKGE