Recombinant human MNDA protein

MNDA, also known as myeloid cell nuclear differentiation antigen, is detected only in nuclei of cells of the granulocyte-monocyte lineage. This protein may act as a transcriptional activator/repressor in the myeloid lineage and plays a role in the granulocyte/monocyte cell-specific response to interferon. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. Recombinant MNDA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Myeloid cell nuclear differentiation antigen, PYHIN3, Myeloid cell nuclear differentiation antigen

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.9 kDa (427aa) confirmed by MALDI-TOF

Additionnal Information

MNDA, Myeloid cell nuclear differentiation antigen, PYHIN3, Myeloid cell nuclear differentiation antigen, ATGP0450-10 µg, ATGP0450-20 µg, ATGP0450-50 µg, ATGP0450-100 µg, ATGP0450-250 µg, ATGP0450-500 µg, ATGP0450-1 mg, ATGP0450-10, ATGP0450-20, ATGP0450-50, ATGP0450-100, ATGP0450-250, ATGP0450-500, ATGP0450-1

References & Citations

Burrus GR., et al. (1992) J. Cell. Biochem. 48 (2) :190-202.; ; Briggs RC., et al. (2005) J. Cell. Biochem. 95 (2) :293-301.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002423

Uniprot Accession Number

P41218

Species

Human

AA Sequence

MVNEYKKILL LKGFELMDDY HFTSIKSLLA YDLGLTTKMQ EEYNRIKITD LMEKKFQGVA CLDKLIELAK DMPSLKNLVN NLRKEKSKVA KKIKTQEKAP VKKINQEEVG LAAPAPTARN KLTSEARGRI PVAQKRKTPN KEKTEAKRNK VSQEQSKPPG PSGASTSAAV DHPPLPQTSS STPSNTSFTP NQETQAQRQV DARRNVPQND PVTVVVLKAT APFKYESPEN GKSTMFHATV ASKTQYFHVK VFDINLKEKF VRKKVITISD YSECKGVMEI KEASSVSDFN QNFEVPNRII EIANKTPKIS QLYKQASGTM VYGLFMLQKK SVHKKNTIYE IQDNTGSMDV VGSGKWHNIK CEKGDKLRLF CLQLRTVDRK LKLVCGSHSF IKVIKAKKNK EGPMNVN