Recombinant human GTF2B protein

GTF2B, also known as transcription factor II B, is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. It localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. The protein serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. It is involved in the selection of the transcription start site. Recombinant GTF2B protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

General transcription factor IIB, TF2B, TFIIB, General transcription factor IIB General transcription factor TFIIB, GTF2B, RNA polymerase II transcription factor IIB, S300 II, TF IIB, Transcription initiation factor IIB.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

36.9 kDa (336aa) confirmed by MALDI-TOF

Additionnal Information

GTF2B, General transcription factor IIB, TF2B, TFIIB, General transcription factor IIB General transcription factor TFIIB, GTF2B, RNA polymerase II transcription factor IIB, S300 II, TF IIB, Transcription initiation factor IIB., ATGP0449-10 µg, ATGP0449-20 µg, ATGP0449-50 µg, ATGP0449-100 µg, ATGP0449-250 µg, ATGP0449-500 µg, ATGP0449-1 mg, ATGP0449-10, ATGP0449-20, ATGP0449-50, ATGP0449-100, ATGP0449-250, ATGP0449-500, ATGP0449-1

References & Citations

Ing N.H., et al. (1992) J. Biol. Chem. 267:17617-17623.; ; Bushnell, David A, et al. (2004) Science. 303 (5660) :983-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 6.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001505

Uniprot Accession Number

Q00403

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF PTDFKFDTPV DKLPQL