Recombinant human GTF2B protein
GTF2B, also known as transcription factor II B, is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. It localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. The protein serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. It is involved in the selection of the transcription start site. Recombinant GTF2B protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
36.9 kDa (336aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ing N.H., et al. (1992) J. Biol. Chem. 267:17617-17623.; ; Bushnell, David A, et al. (2004) Science. 303 (5660) :983-8.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM MES buffer (pH 6.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001505
Uniprot Accession Number
Q00403
Species
Human
AA Sequence
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