Recombinant human Frataxin protein

Frataxin, also known as FXN, is an iron binding protein which belongs to FRATAXIN family. It is an anti-apoptotic protein which prevents mitochondrial damage, reactive oxygen species production and act as either an iron chaperone or an iron storage protein. Frataxin is localized to the mitochondrion and regulating mitochondrial iron transport and respiration. Defects in this protein cause Friedreich ataxia. Recombinant Frataxin protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CyaY, FA, FARR, FRDA, X25, FXN, Friedreich ataxia protein.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

21.1 kDa (190aa) confirmed by MALDI-TOF

Additionnal Information

Frataxin, ATGP0448-10 µg, ATGP0448-20 µg, ATGP0448-50 µg, ATGP0448-100 µg, ATGP0448-250 µg, ATGP0448-500 µg, ATGP0448-1 mg, ATGP0448-10, ATGP0448-20, ATGP0448-50, ATGP0448-100, ATGP0448-250, ATGP0448-500, ATGP0448-1

References & Citations

Campuzano V., et al. (1996) Science. 271 (5254) :1423-7. ; ; Shan Y., et al. (2007) Hum Mol Genet. 16 (8) :929-41. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 20% glycerol, 0.1M NaCl 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_000135.2

Uniprot Accession Number

Q16595

Species

Human

AA Sequence

LRTDIDATC TPRRASSNQR GLNQIWNVKK QSVYLMNLRK SGTLGHPGSL DETTYERLAE ETLDSLAEFF EDLADKPYTF EDYDVSFGSG VLTVKLGGDL GTYVINKQTP NKQIWLSSPS SGPKRYDWTG KNWVYSHDGV SLHELLAAEL TKALKTKLDL SSLAYSGKDA