Recombinant human Thymidylate synthase/TYMS protein

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-Fu) . Resistance or lack of response to 5-Fu is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

HST422, TMS, TS, d TMP synthase, EC 2.1.1.45, MGC88736, Thymidylate synthase, Thymidylate synthetase, Tsase, TYMS, TYMS protein, Tyms thymidylate synthetase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.8 kDa (333aa) confirmed by MALDI-TOF

Additionnal Information

Thymidylate synthase, HST422, TMS, TS, d TMP synthase, EC 2.1.1.45, MGC88736, Thymidylate synthase, Thymidylate synthetase, Tsase, TYMS, TYMS protein, Tyms thymidylate synthetase, ATGP0446-10 µg, ATGP0446-20 µg, ATGP0446-50 µg, ATGP0446-100 µg, ATGP0446-250 µg, ATGP0446-500 µg, ATGP0446-1 mg, ATGP0446-10, ATGP0446-20, ATGP0446-50, ATGP0446-100, ATGP0446-250, ATGP0446-500, ATGP0446-1

References & Citations

Chiu TH., et al. (2009) Anticancer Res. 29 (11) :4503-11. ; ; Pena MM., et al. (2009) J Biol Chem. 284 (46) :31597-607.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001062

Uniprot Accession Number

P04818

Species

Human

AA Sequence

MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV