Recombinant human ERK2/MAPK1 protein
MAPK1, also known as ERK (extracellular signal-regulated kinase), acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Recombinant human MAPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
43.5 kDa (380aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hu S., et al. (2009) Cell. 139 (3) :610-22. ; ; McGargill MA., et al. (2009) J Immunol. 183 (8) :4838-42.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_620407
Uniprot Accession Number
P28482
Species
Human
AA Sequence
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